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and Research Laboratory |
Serology |
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HIV
Antibody ELISA:
It is a screening test for HIV antibodies detection
and with these assays, the antibodies are generally
detectable within 21 days after HIV infection in humans
(range of 9 to 34 days). The efficient HIV testing
algorithm for the diagnosis of HIV infection is determined
based on the sensitivity and specificity of the screening
assays and the established algorithm is commonly used.
Although HIV Western blot is one of the confirmatory
tests, but this is not commonly practiced in developing
countries.
HIV
Western Blot:
An antibody test with very high specificity is required
as a supplemental/confirmatory assay. This assay
shows the interpreter precisely, which HIV antigens
have elicited an antibody response in the patient.
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Diagnosis of Other Infections:
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Diagnosis of Sexually
Transmitted Infections such as Herpes Simplex Virus-2
viral antibody by ELISA, Syphilis by RPR and TPPA/TPHA. |
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Diagnosis of Hepatitis Viruses
by ELISA (Anti-HCV and HBsAg). |
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Diagnosis of OIs such as antibodies
to Toxoplasma gondii (differentiation of IgG and IgM
is also done); detection of Cryptococcus neoformans
antigen in serum or CSF is done by rapid Latex Agglutination
Test. |
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Typhoid diagnosis done by WIDAL
testing - for Salmonella typhi and Salmonella paratyphi
infections. |
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For neuro-syphilis RPR is done
in CSF. |
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Detuned Assay for Recent HIV Infection:
Detuned ELISA (less sensitive assay) for HIV-1 incidence
estimation is widely applied as one of the epidemiological
research tools. This assay has been developed by researcher,
Dr.Bharat Parekh, PhD in Centre for Disease Control and Prevention
(CDC), Atlanta US.
Non-NAT
Plasma Viral Load Assay:
We have also successfully standardized a low cost HIV viral
load assay, which quantifies reverse trancriptase activity
of HIV using an ExaVir load assay kit. Currently, this assay
is being evaluated for the use in resource-limited countries
in collaboration with Prof. Suzanne Crowe, Burnet Institute,
Melbourne, Australia.
The laboratory is equipped with 2 ELISA Readers, 2 ELISA Washers,
Rotator, Rocker, Centrifuges, Biological Incubator, and Electronic
Pipettes. |
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| ELISA
Plate Washer |
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| ELISA
Plate Reader |
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